Today's Veterinary Practice

JUL-AUG 2015

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TodAy's VeTerinAry PrAcTice | July/August 2015 | tvpjournal.com in-cLinic HeMAToLoGy Peer reviewed 48 to an intense demand for infammatory cells in peripheral tissues. Reactive lymphocytes are nonspecifc indicators of antigenic stimulation. They are characterized by a slightly larger size (in some cases, approximately the size of a neutrophil), increased amounts of cytoplasm that can have enhanced cytoplasmic basophilia, prominent perinuclear clear zone, a few small clear punctate vacuoles, and/or small magenta granules (Figure 16); nucleoli should not be present. WBC inclusions may indicate an infectious agent. distemper inclusion bodies can be visualized in leukocytes and erythrocytes (Figure 13); other relevant infectious agents include tick-borne diseases caused by Anaplasma species (Figure 17). rarely bacteria can be seen within neutrophils or monocytes in septic patients. High power examination of the feathered edge is recommended to optimize identifcation of infectious agents. Although leukocyte morphology is often distorted, the concentration of cells usually allows visualization of rare agents. infectious agents can be identifed in patients with quantitatively normal cBc results. Lymphoblasts (Figure 18), mast cells (Figure 19), and malignant histiocytes are abnormal cells seen with some frequency but not specifcally identifed by analyzers. • Immature lymphocytes, in small numbers, are occasionally seen in septic patients; therefore, a pathologist should always review smears containing immature lymphocytes. • Mast cells are often easiest to identify by examining the feathered edge of the smear, but FIGURE 19. Feline blood flm with 2 mast cells and a basophil on the feathered edge; a distorted cell lacking discernable margins is also present. Note presence of dark granular debris that is artifact from slide preparation (arrow). Wright-Giemsa stain; magnifcation, 1000×. FIGURE 18. Numerous large lymphocytes with occasional visible nucleoli (arrows) present in a canine patient with acute lymphoblastic lymphoma. Wright-Giemsa stain; magnifcation, 1000×. FIGURE 17. Variably shaped Anaplasma morulae ( arrows ) within segmented neutrophils are present. The patient was antibody negative at time of organism identifcation. Note that morulae are lighter than neutrophil chromatin. Wright-Giemsa stain; magnifcation, 1000×. FIGURE 16. Canine blood flm with 2 reactive lymphocytes with increased size, cytoplasmic basophilia, and perinuclear clearing. Wright- Giemsa stain; magnifcation, 1000×.

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