Today's Veterinary Practice

SEP-OCT 2015

Today's Veterinary Practice provides comprehensive information to keep every small animal practitioner up to date on companion animal medicine and surgery as well as practice building and management.

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tvpjournal.com | September/October 2015 | TOday'S VeTerinary PracTice ParaSiTOlOgy eXPerTiSe FrOM THe ncVP Peer reviewed 55 • centrifugal fecal flotation documented eggs of Trichuris species in 26% of feral cat samples, while enzyme-linked immunosorbent assay (eliSa) detected Trichuris species antigen in 39% of fecal samples. • These data underscore both the importance of this parasite in cats in this Florida region and the utility of fecal eliSa for identifying whipworm infections. 15 Evaluating Schistosoma mansoni Point-of- Care Immunodiagnostic Tests for Detection of Heterobilharzia americana Antigen in Dogs Jessica Rodriguez, DVM, Texas A&M; University • Heterobilharzia americana is a schistosome (trematode) of raccoons that occasionally infects dogs and horses (Figure 4). 16 diagnosis is generally made by identification of eggs in fecal saline sedimentation or by fecal polymerase chain reaction, but intermittent egg shedding may make these tests insensitive. • This presentation provided information from a study that evaluated the ability of point-of- care immunodiagnostic tests designed for the human parasite, Schistosoma mansoni, to detect H americana. • While the assay was successful at detecting infection in some samples, diagnosis was most likely determined when this approach was combined with the aforementioned microscopic and molecular techniques. IN SUMMARY as shown in this brief review, the presentations at the aaVP annual Meeting were focused on the future, highlighting novel parasites, geographic distributions, and diagnostic techniques. The aaVP continues to strive to provide evidence-based recommendations that clinicians can put into practice. aaVP = american association of Veterinary Parasitologists; eliSa = enzyme-linked immunosorbent assay; iSeP = international Symposium on ectoparasites of Pets; liWc = livestock insect Workers conference References 1. crossland na, Hanks cr, Ferguson Ja, et al. First report of Cylicospirura felineus in a feral domestic shorthair cat in north america. J Fel Med Surg 2015; Open Reports 1-4. 2. conboy g, Stewart T, O'Brien S. Treatment of E boehmi infection in a mixed-breed dog using milbemycin oxime. JAHAA 2013; 49(3):204-209. 3. Veronesi F, Morganti g, di cesare a, et al. a pilot trial evaluating the effcacy of a 10% imidacloprid/2.5% moxidectin spot-on formulation in the treatment of natural nasal capillariasis in dogs. Vet Parasitol 2014; 200:133-138. 4. Otranto d, giannelli a, latrofa MS, et al. canine infections with Onchocerca lupi nematodes, United States, 2011-2014. Emerg Infect Dis 2015; 21(5):868-871. 5. Boyce W, Shender l, Schultz l, et al. Survival analysis of dogs diagnosed with canine peritoneal larval cestodiasis (Mesocestoides spp.). Vet Parasitol 2011; 180(3-4):256-261. 6. Stockdale-Walden Hd, Slapcinsky J, Qvarnstrom y, et al. Angiostrongylus cantonensis in introduced gastropods in southern Florida. J Parasitol 2015; 101(2):156-159. 7. Bouchard c, leonard e, Koff JK, et al. The increasing risk of lyme disease in canada. Can Vet J 2015; 56(7):693-699. 8. Wang a, ruch-gallie r, Scorza V, et al. Prevalence of Giardia and Cryptosporidium species in dog park attending dogs compared to non-dog park attending dogs in one region of colorado. Vet Parasitol 2012; 184(2-4):335-340. 9. Smith aF, Semeniuk ca, Kutz SJ, Massolo a. dog-walking behaviours affect gastrointestinal parasitism in park-attending dogs. Parasit Vectors 2014; 7:429. 10. dantas-Torres F, latrofa MS, annoscia g, et al. Morphological and genetic diversity of Rhipicephalus sanguineus sensu lato from the new and Old Worlds. Parasit Vectors 2013; 6:213. 11. cicuttin gl, Tarragona el, de Salvo Mn, et al. infection with Ehrlichia canis and Anaplasma platys (rickettsiales: anaplasmataceae) in two lineages of Rhipicephalus sanguineus sensu lato (acari: ixodidae) from argentina. Ticks Tick Borne Dis 2015; in press (doi:10.1016/j.ttbdis.2015.06.006). 12. Mertins JW, Moorhouse aS, alfred JT, Hutcheson HJ. Amblyomma triste (acari: ixodidae): new north american collection records, including the frst from the United States. J Med Entomol 2010; 47(4):536-542. Figure 4. Heterobilharzia americana: Fecal sedimentation with saline demonstrating H americana eggs from a dog; these eggs are approximately 74 to 113 mcm by 60 to 80 mcm and contain fully formed miracidia. Intermittent egg shedding makes fecal sedimentation an insensitive test for diagnosing this parasite. Courtesy National Center for Veterinary Parasitology Modifed Knott's Technique A stepwise approach to the modifed Knott's technique is described in Heartworm Diagnostics: What Do the latest American Heartworm society Canine guidelines tell us? (November/ December 2014 issue), available at tvpjournal.com .

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