Today's Veterinary Practice

JUL-AUG 2011

Today's Veterinary Practice provides comprehensive information to keep every small animal practitioner up to date on companion animal medicine and surgery as well as practice building and management.

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ADVANCES IN STEM CELL THERAPY | UÊ iÃk UÊ ÕÃVli UÊ iÀÛiÊÌiÃÃÕi UÊ oni UÊ im>Ìo«oiiÌiV-ÃÕ««oÀÌin}ÊÃÌÀom>° In vitro, AD-SCs have demonstrated pluripotent capabilities to differentiate into a variety of nonme- sodermally derived tissues, including hepatic, pan- creatic, and keratocytic tissue, and to be effective in skin anti-aging and tissue regeneration, cardiovas- cular muscle and vascular tissue repair, rheumatoid arthritis, diabetes, and other diseases.ÈÈ-Çx oÀÊm>nÞÊÞi>ÀÃÊinÊhÕm>nÊmi`iVini]ÊÃÌÕ`iiÃÊh>ÛiÊ focused on bone marrow-derived mesenchymal and hematopoietic undifferentiated cells. It is well documented that bone marrow possesses very few true mesenchymal stem cells, compared to AD-SCs, which are becoming the primary tissue source in VliniV>lÊ >««liV>ÌionÃ°Ê `i«oÃiÊiÃÊ>ÊVom«liÝÊVillÕ- lar–vascular tissue that is not only easier to harvest, but offers markedly higher nucleated, undifferenti- ated stem cell counts than bone marrow.ÇÈ Research has shown as much as 500 to 1000 times as many TECHNOLOGY FOR STEM CELL & PLASMA CONCENTRATE HARVESTING Stem Cell Harvesting $VSSFOUMZ DPNQBOJFT QSPWJEF TUFN DFMM UFDIOPMPHZ GPS WFUFSJOBSZ NFEJDJOF Vet-Stem WFU TUFN DPN UIF GJSTU DPNQBOZ UP DPNNFSDJBMMZ PGGFS TUFN DFMM VTF QSPWJEFT B QSBDUJDBM iBT OFFEFEu BQQSPBDI 7FUFSJOBSJBOT DBO SFNPWF GBU GSPN BO BOJNBM BOE TIJQ JU PWFSOJHIU UP UIF 7FU 4UFN MBCPSBUPSZ 5IFSF UIF GBU TBNQMF JT QSPDFTTFE CZ DIFNJDBM EJHFTUJPO JTPMBUJPO BOE DPODFOUSBUJPO UIFO SFUVSOFE UP UIF WFUFSJOBSJBO GPS JOKFDUJPO JOUP UIF BOJNBM T JOKVSZ TJUF MediVet America NFEJWFU BNFSJDB DPN PGGFST UIF BCJMJUZ UP EP TUFN DFMM QSPDFEVSFT BU QPJOU PG DBSF XJUIJO DMJOJDBM TVSHJDBM GBDJMJUJFT #PUI DPNQBOJFT IBWF SFQPSUFE GBWPSBCMF SFTVMUT JO UIFJS USFBUNFOU QSPUPDPMT BOE SFQPSUFE DBTF TUVEJFT Plasma Concentrate Harvesting 7BSJPVT QPSUBCMF DPNNFSDJBM DFOUSJGVHBUJPO VOJUT FYJTU UIBU QSPDFTT CMPPE TBNQMFT SFTVMUJOH JO QMBUFMFU SJDI QMBTNB DPODFOUSBUFT 5IF POF VTFE GPS PVS JOWFTUJHBUJPO XBT UIF QBUFOUFE Harvest Technologies IBSWFTUUFDI DPN 4NBSU 13F1 DFOUSJGVHBUJPO TZTUFN 5IJT TZTUFN VTFT B TUFSJMJ[FE CMPPE DPMMFDUJPO LJU UIBU BMMPXT JO PGGJDF QIMFCPUPNZ BOE QSPDFTTJOH JO B UBCMFUPQ CJEJSFDUJPOBM DFOUSJGVHBUJPO VOJU *U JT DBQBCMF PG DPOTJTUFOUMZ DPODFOUSBUJOH UP UJNFT PS NPSF UIF QBUJFOU T DJSDVMBUJOH MFWFM PG QMBUFMFUT BDIJFWJOH UIF OFFEFE UIFSBQFVUJD MFWFM PG )%131 References 1. Vet Stem case studies: vet-stem.com/equine/casestudies.php, vet-stem.com/ smallanimal/casestudies.php 2. MediVet America case studies: medivet-america.com/casestudies.html 3. Harvest Technologies product information: harvesttechnologies.com/products/ smartprepmain.html mesenchymal and stromal vascular stem-like cells iÝiÃÌÊinÊ>`i«oÃiÊ>ÃÊVom«>Ài`ÊÌoÊLoniÊm>ÀÀoÜ°ÇÇ-Ç9 further information on ongoing stem cell research, go to todaysveterinarypractice.com. STEM CELL HARVESTING & ADMINISTRATION The ability of AD-SCs to support and serve as a cell reservoir for connective tissue and joint repair is the basic theory for their use in joint regenerative medicine. Autologous AD-SC therapy involves: £°Ê >ÀÛiÃÌin}Êv>ÌÊvÀomÊÌhiÊ>Û>il>LliÊÃiÌià 2. Isolating the stem and regenerative cells 3. Administering the cells back to the patient. In veterinary practice, these cells are typically found in the adipose deposits near the tail (horses) and from the ventral fat pad (dogs). In these cases, fat is either lipoaspirated80 or, if taken surgically, en bloc, morselized to permit separation of the stem– ÃÌÀom>lÊiliminÌÃÊÜiÌhinÊÌhiÊ>`i«oÃiÊVom«liÝ°Ê vÌiÀÊ «Ài«>À>Ìion]ÊÌhiÃÊÌiÃÃÕi]ÊVomLini`ÊÜiÌhÊ *,*]ÊV>nÊ then be injected during open surgical intervention or by guided injection. It is important to understand that undifferenti- ated stem/stromal cells must adhere to other cells ­Vill-Ìo-VillÊVonÌ>VÌ®ÊoÀÊÌoÊiÝÌÀ>VillÕl>ÀÊm>ÌÀiÝÉ«iÀi- vascular tissues in order to proliferate effectively. Within an injured or degenerative site, the stem/ ÃÌÀom>lÊVillÊv>ÌiÊiÃÊVonÌÀolli`ÊLÞÊ>ÊVom«liÝÊÃiÌÊovÊ physical (cell-to-cell) and chemical signals (para- crine-autocrine effects) dictated by the cellular and chemical microenvironment (niche).81 ÕÀÌhiÀ]Ê ÌhiÃiÊ VillÃÊ >ÀiÊLiliiÛi`Ê ÌoÊ«ÀoliviÀ>ÌiÊ and differentiate from signaling based on the envi- ronment in which they are placed. Therefore, if AD-SCs are placed within and adherent to damaged connective tissue, uncommitted progenitor and stem/stromal elements within the AD-SC graft are activated and differentiate toward the specific con- nective tissue lineages for growth and repair. CONCLUSION Stem cell therapy in regenerative veterinary medi- cine is a viable option for the equine as well as the small animal veterinarian, offering a safe and clin- ically effective tool for the clinician to assist in his/ her treatment of the animal with difficult wounds or unresolved musculoskeletal or joint pain. Q "% 4$ BEJQPTF EFSJWFE TUFN TUSPNBM DFMM )%131 IJHI EFOTJUZ QMBUFMFU SJDI QMBTNB Go to TodaysVeterinaryPractice.com for further information on how adipose-derived stem cells were originally introduced for use in medicine as well as details regarding current research taking place. July/August 2011 Today’s Veterinary Practice 27 Ê oÀÊ

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